EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.68 | - |
Mycobacterium tuberculosis |
2.5.1.86 | - |
Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.68 | crystallization using sparse matrix screens and vapor diffusion, structure of apo Rv1086 with citronellyl diphosphate bound and with the product mimic (2E,6E)-farnesyl diphosphate bound. The maximal reaction rate of the wild-type enzyme is 10fold greater than that of the mutant enzyme | Mycobacterium tuberculosis |
2.5.1.68 | in complex with citronellyl diphosphate and with product mimic (E,E)-farnesyl diphosphate | Mycobacterium tuberculosis |
2.5.1.86 | crystallization using sparse matrix screens and vapor diffusion, structures of Rv2361c in the apo form, with isopentyl diphosphate bound and with a substrate analogue, citronellyl diphosphate | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.68 | L84A | potentially important in limiting chain length. The mutant L84A is not expressed in a soluble form | Mycobacterium tuberculosis |
2.5.1.68 | L84A/L85F | mutant enzyme has a Km-value for geranyl diphosphate of 0.15 mM compared to 0.038 mM for wild-type Rv108612 | Mycobacterium tuberculosis |
2.5.1.68 | L84A/L85F | well expressed and active. Mutant protein is capable of synthesizing prenyl diphosphates containing 20 carbon atoms when geranyl diphosphate is used as the allylic substrate and 50 carbon atoms when EE-farnesyl diphosphate is used as the allylic substrate | Mycobacterium tuberculosis |
2.5.1.68 | V107G | inactive | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.68 | 0.15 | - |
geranyl diphosphate | mutant L84A/L85F | Mycobacterium tuberculosis | |
2.5.1.68 | 0.15 | - |
geranyl diphosphate | pH 7.9, 37°C, mutant enzyme L84A/L85F | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis | the enzyme is involved in the biosynthesis of decaprenyl phosphate, which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | the enzyme is involved in the biosynthesis of decaprenyl phosphate, which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.68 | Mycobacterium tuberculosis | P9WFF5 | - |
- |
2.5.1.68 | Mycobacterium tuberculosis H37Rv | P9WFF5 | - |
- |
2.5.1.86 | Mycobacterium tuberculosis | P9WFF7 | - |
- |
2.5.1.86 | Mycobacterium tuberculosis H37Rv | P9WFF7 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + trans,octacis-decaprenyl diphosphate | mechanism: 1. the C5 unit of isopentenyl diphosphate adds to the a-end of the polyisoprene polymer, 2. in processive enzymes such as Rv2361c, the nascent chain (C15 to C45) moves to the polymer site displacing the diphosphate, 3. the empty isopentenyl diphosphate site is then reloaded and another C5 unit is added in an iterative fashion until the physiologically relevant chain length is generated | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.68 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | ? + diphosphate | no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms | ? | |
2.5.1.68 | (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | ? + diphosphate | no substrate for wild-type enzyme. Mutant L84A/L85A synthesizes prenyl diphosphates containing 50 carbon atoms | ? | |
2.5.1.68 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | (2Z,6E)-farnesyl diphosphate + diphosphate | product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms | ? | |
2.5.1.68 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | (2Z,6E)-farnesyl diphosphate + diphosphate | product of wild-type.Mutant L84A/L85A synthesizes prenyl diphosphates containing 20 carbon atoms | ? | |
2.5.1.68 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
2.5.1.68 | geranyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + (2Z,6E)-farnesyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the enzyme is involved in the biosynthesis of decaprenyl phosphate, which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan | Mycobacterium tuberculosis | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the enzyme is involved in the biosynthesis of decaprenyl phosphate, which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan | Mycobacterium tuberculosis H37Rv | 7 diphosphate + (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E)-decaprenyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.68 | dimer | gel filtration and crystallization data | Mycobacterium tuberculosis |
2.5.1.86 | dimer | crystal structure suggests that Rv2361c is a dimer | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.68 | Rv1086 | - |
Mycobacterium tuberculosis |
2.5.1.86 | Rv2361c | - |
Mycobacterium tuberculosis |